Device

Part:BBa_M36124:Design

Designed by: Daniel Hart, Gustavo Garcia, Raymond Serrano   Group: Stanford BIOE44 - S11   (2015-11-05)


Glucose-6-Phosphate Isomerase for manipulation of glycolysis in yeast


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 793
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 793
    Illegal BglII site found at 61
    Illegal BglII site found at 409
    Illegal BamHI site found at 702
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 793
    Illegal AgeI site found at 433
    Illegal AgeI site found at 1123
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 17


Design Notes

We edited the genetic sequence to remove repeats greater than 10 base pairs in length, so that it could be synthesized and replicated easily. All mutations introduced were silent to maintain an identical function of the wild type protein. This was to allow better comparison of the effects of overexpression.


Source

The part was derived from a uniprot sequence of S cerevisiae (baker's yeast), strain ATCC 204508 / S288c.

References